Hello, in Camarinha-Silva et al. 2014, the mean number of sequences per sample was 7682 ± 484, totalling 1 459 615 usable paired-end sequence reads. In your study, after quality filtering, a total of 782,683 paired-end reads, with an average of 41,194 reads per sample (a minimum of 30,101 reads and a maximum of 62,232 reads) were obtained. So your average reads is >5 folds more than previous stud...