Sir Want to know how to cluster and assemble the sequences. We are having ezRAD data for 12 genotypes. Now how to proceed to make a reference assembly. Whether data for all genotypes will be used for reference preparation or data from a specific genotype has to be assemble. Plz guide us, Regards Sushil

Hi, I am a little confused by this idea of pooled vs unpooled libraries here. By 'pooled', do you mean all the 4 individuals in each library share just one barcode (ie total 2 barcodes), whereas the 8 'unpooled' ones each have one barcode (ie total 8 barcodes required)? If this is the case, how do you demultiplex the 4 individuals in each of the 2 libraries?